Anti-Human GPRC5D Monoclonal Antibody for Flow Cytometry - Q9NZD1

Core Product Specifications and Parameters

Biological Background: GPRC5D Function and Localization

• GPRC5D (G protein-coupled receptor family C group 5 member D) is a member of the GPCR superfamily, predicted to possess a 7-transmembrane helical domain architecture characteristic of signal-transducing receptors.
• The receptor is implicated in the regulation of hard keratin expression and likely plays a role in the development of hair and nails.
• Subcellular fractionation and annotation indicate localization at the cell membrane, consistent with its function as a surface receptor.
• GPRC5D exhibits a restricted tissue expression pattern: it is highly expressed in pancreas, moderately in kidney, small intestine, spleen, and testis, weakly in lung, colon, leukocyte, prostate, and thymus, and absent in brain, heart, liver, placenta, skeletal muscle, and ovary.
• Alternative splicing generates multiple isoforms; the canonical isoform has a calculated molecular weight of approximately 38.8 kDa, while experimental data suggest bands at 34, 36, and 38 kDa, possibly reflecting post-translational modifications or isoform-specific processing.
• Relevant UniProt keywords include: 3D-structure, alternative splicing, cell membrane, G protein-coupled receptor, membrane, transducer, transmembrane, transmembrane helix, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1-100), ensuring specificity for the target sequence; consider epitope accessibility in native versus denaturing conditions.
• This conjugate is directly compatible with flow cytometry; titrate the antibody for optimal signal-to-noise ratio using GPRC5D-positive and -negative cell lines.
• Include appropriate isotype controls and viability dyes to exclude non-specific binding and dead cells.
• Validate staining in cell systems that endogenously express GPRC5D (e.g., pancreatic or renal cell lines) or use transfected overexpression models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and life science researchers with streamlined access to this anti-GPRC5D monoclonal antibody, offering validated reagents for flow cytometry applications. Complementing this product, our portfolio includes monoclonal and polyclonal antibodies, optimized antibody pairs, and ancillary reagents to support GPRC5D-related studies in dermatology and epithelial biology. From single-vial orders to bulk custom sourcing, CamelBio bridges the gap between target discovery and translational research.