ABflo 647 Anti-Human GP6/GPVI Monoclonal Antibody for Flow Cytometry - Q9HCN6

Core Product Specifications and Parameters

Biological Background: GP6/GPVI Function and Localization

• Platelet glycoprotein VI (GP6), also known as GPVI or BDPLT11, is a critical collagen receptor on platelets, mediating collagen-induced platelet adhesion and activation, and contributing to arterial and venous thrombus formation.
• The receptor signaling pathway involves the FcR gamma-chain, Src family kinases (likely FYN or LYN), SYK, and the adapter protein LAT, leading to the activation of PLCG2.
• Expressed specifically in megakaryocytes and platelets, with no significant expression in other tissues.
• Localized at the cell membrane, featuring immunoglobulin-like domains, an extracellular region, and a transmembrane helix.
• Post-translational modifications include disulfide bond formation and glycosylation; undergoes alternative splicing to produce multiple isoforms.
• Belongs to the immunoglobulin superfamily of receptors, characterized by immunoglobulin-like domains, and plays a central role in hemostasis and blood coagulation.
• UniProt keywords include: Blood coagulation, Hemostasis, Receptor, Immunoglobulin domain, Transmembrane helix, Disease variant, and others.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 21–267), suggesting the antibody likely recognizes an extracellular epitope, making it suitable for flow cytometry on non-permeabilized platelets.
• For flow cytometry, isolate human platelets using gentle methods (e.g., platelet-rich plasma) to minimize pre-activation; consider using PGI2 or apyrase to maintain resting state.
• Include appropriate isotype controls and Fc receptor blocking (e.g., human Fc block) to reduce non-specific binding, as platelets express FcγRIIa.
• Validate specificity by testing on GP6-deficient platelets or using blocking peptides; assess potential cross-reactivity with other immunoglobulin superfamily members.
• The ABflo 647 conjugate is bright and photostable, suitable for multi-color panels; titrate the antibody to determine the optimal staining concentration for your conditions.
• During data analysis, gate on single platelets by FSC/SSC and consider monitoring activation markers (e.g., PAC-1 or CD62P) if studying collagen-induced responses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a single sourcing channel for IVD raw materials, offering diagnostic manufacturers and research institutes streamlined access to validated antibodies, bulk reagents, and custom conjugation services. For platelet biology and thrombosis research, we supply a range of monoclonal antibodies targeting platelet glycoproteins such as GP6/GPVI to support flow cytometry and functional assay development. Our team also assists with sourcing rare targets and optimizing immunoassay components, bridging the gap from concept to clinical application.