Anti-Human Glypican 3 (GPC3) Monoclonal Antibody for Flow Cytometry - P51654

Core Product Specifications and Parameters

Biological Background: Glypican-3 Function and Localization

• Glypican-3 (GPC3) is a cell surface proteoglycan attached to the plasma membrane via a GPI anchor (PubMed:14610063). Related references: PMID:14610063
• It negatively regulates Hedgehog signaling by competing with PTC1 for hedgehog proteins and promoting internalization/degradation of SHH.
• GPC3 positively regulates the canonical Wnt pathway by binding to Frizzled receptors and enhancing Wnt ligand binding (PubMed:16227623, PubMed:24496449). Related references: PMID:16227623 PMID:24496449
• The protein inhibits DPP4 dipeptidyl peptidase activity (PubMed:17549790) and also binds CD81 to modulate HHEX transcriptional repression. Related references: PMID:17549790
• During embryogenesis, GPC3 is essential for limb patterning, skeletal development, and renal branching morphogenesis by modulating BMP and FGF signaling.
• Tissue expression: high in placenta (confirmed at protein level), lung, liver, and kidney, correlating with its roles in organogenesis and tumor biology.
• Post-translational modifications include cleavage, heparan sulfate attachment, and phosphorylation; alternative splicing yields isoforms observed at 59–68 kDa (canonical mass 65.6 kDa).
• Keywords: Cell membrane, GPI-anchor, Glycoprotein, Heparan sulfate, Proteoglycan, Disease variant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 481–580) near the GPI-attachment site; the antibody may recognize both full-length and cleaved forms, but validate in your specific sample system.
• As a direct conjugate, this antibody is ready-to-use for flow cytometry without secondary staining; consider titrating the antibody for optimal signal-to-noise.
• Given GPC3's cell surface localization, live-cell staining protocols may be employed; fixation/permeabilization conditions should be empirically tested if also probing intracellular epitopes.
• For multiplex flow panels, ensure minimal spectral overlap with other fluorophores; excites at 488 nm and emits at ~520 nm.
• No cross-reactivity data with other species is provided; expression in mouse or rat should be confirmed if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide a comprehensive portfolio of IVD raw materials, including this GPC3 monoclonal antibody for flow cytometry applications. Beyond individual reagents, we offer customized sourcing for validated antibody pairs, bulk ancillary reagents, and rare target antigens to accelerate your assay development from concept to clinic. Contact us to discuss your specific GPC3 detection needs or broader immunodiagnostic material requirements.