Anti-Human Galectin-3 Monoclonal Antibody for Flow Cytometry (Intracellular) - P17931

Core Product Specifications and Parameters

Biological Background: Galectin-3 Function and Localization

• Galectin-3, encoded by the LGALS3 gene, is a multifunctional protein also known as 35 kDa lectin, carbohydrate-binding protein 35 (CBP35), galactoside-binding protein (GALBP), IgE-binding protein, L-31, laminin-binding protein, lectin L-29, and Mac-2 antigen.
• It functions as a galactose-specific lectin that binds IgE and mediates interactions with integrins, contributing to endothelial cell migration and columnar epithelial cell differentiation during embryogenesis.
• In the nucleus, galectin-3 acts as a pre-mRNA splicing factor, while in the cytoplasm, it participates in the recognition of damaged endomembranes and coordinates autophagy by recruiting ATG16L1 and BECN1 together with TRIM16.
• It plays critical roles in acute inflammatory responses, including neutrophil activation and adhesion, chemoattraction of monocytes/macrophages, opsonization of apoptotic neutrophils, and mast cell activation.
• The protein shuttles between the cytoplasm, nucleus, and extracellular space, being secreted via a non-classical pathway despite lacking a signal peptide.
• Galectin-3 undergoes post-translational modifications such as acetylation and phosphorylation, and contains disulfide bonds, which may regulate its lectin activity and stability.
• It is predominantly expressed in the colonic epithelium, activated macrophages, and fetal membranes, highlighting its importance in immune defense and tissue remodeling.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein covering the full-length human galectin-3 (aa 1–250); it is expected to recognize both cytoplasmic and nuclear pools of the protein.
• For intracellular flow cytometry (FC intra), optimize permeabilization and fixation protocols to ensure antibody access to galectin-3 in various subcellular compartments.
• As galectin-3 can also be secreted, consider using secretion inhibitors or analyzing extracellular fractions if surface or released protein is of interest; appropriate controls should be included.
• Validate staining specificity using galectin-3 knockout or knockdown cell lines, or pre‑incubate the antibody with the immunogen to confirm signal reduction.
• Antibody concentration and incubation times may require titration for your specific experimental system; test a range of dilutions to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinical application. We provide access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing capabilities, including galectin-3‑related immunoreagents. Whether you need high‑performance antibodies for flow cytometry or bespoke protein supplies, CamelBio is your reliable partner in accelerating diagnostic development.