Anti-Human FcεR1α Monoclonal Antibody () for FC - P12319

Core Product Specifications and Parameters

Biological Background: FcεRIα Function and Localization

• FcεRIα is the alpha subunit of the high-affinity IgE receptor (FcεRI), a tetrameric complex that mediates IgE effector functions on myeloid cells.
• Upon IgE binding and allergen cross-linking, it triggers signaling cascades leading to degranulation, release of vasoactive amines, lipid mediators, and cytokines, driving immediate hypersensitivity reactions.
• The receptor is primarily expressed on mast cells, basophils, and eosinophils, playing a critical role in allergic inflammation and host defense against parasites and venom.
• FcεRIα is a transmembrane glycoprotein located on the cell membrane, containing two extracellular immunoglobulin-like domains, a disulfide-bonded structure, and N-glycosylation sites.
• It is essential for both physiological immune responses and pathological allergic conditions, including asthma and anaphylaxis.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 26–205), making this antibody suitable for detecting surface FcεRIα in live-cell flow cytometry.
• The conjugate provides bright far-red fluorescence; consider using a viability dye to exclude dead cells, as eosinophils and basophils may exhibit autofluorescence.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions, and include appropriate isotype controls.
• For multicolor panels, ensure proper compensation setup, as the signal may spread into adjacent detectors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, purified antigens, and ancillary reagents. This anti-FcεRIα monoclonal antibody is part of our allergy and inflammation target range, enabling high-sensitivity flow cytometry for immune monitoring applications. Through our integrated sourcing, technical, and consulting services, we help accelerate assay development from concept to clinic.