Anti-Human FATP1 () Rabbit Monoclonal Antibody for Flow Cytometry - Q6PCB7

Core Product Specifications and Parameters

Biological Background: FATP1 Function and Localization

• FATP1 (SLC27A1), also known as ACSVL5, FATP-1, long-chain fatty acid transport protein 1, arachidonate--CoA ligase, and very long-chain acyl-CoA synthetase, is a member of the solute carrier family 27. It is subject to alternative splicing, yielding multiple isoforms.
• Core function 1: Mediates cellular import of long-chain fatty acids (LCFA) at the plasma membrane.
  Related references:
  PMID:12556534, PMID:20530735, PMID:21395585
• Core function 2: Also acts as an acyl-CoA ligase, catalyzing ATP-dependent formation of fatty acyl-CoA from LCFA and very-long-chain fatty acids (VLCFA), preventing efflux and promoting net fatty acid accumulation.
• Subcellular localization: Localizes to the cell membrane and endomembrane system, and is present in the cytoplasm, consistent with roles in transport and intracellular fatty acid activation.
• Tissue distribution: Highest expression in muscle and adipose tissue, moderate in small intestine, very low in liver; also detected in brain gray matter.
• Physiological role: Regulates availability of exogenous LCFA for beta-oxidation or triglyceride synthesis in metabolically active tissues, and may play a role in cholesterol metabolism (By similarity).
• Blood-brain barrier: Probably involved in fatty acid transport across the blood-brain barrier, supported by brain expression and functional evidence.
  Related references:
  PMID:21395585

Experimental Guidance and Technical Tips

• The immunogen (amino acids 510–646 of human FATP1) maps to the C-terminal region; this may influence epitope accessibility depending on fixation and permeabilization protocols for intracellular flow cytometry. Consider optimizing these conditions for your specific sample type.
• Because FATP1 is highly expressed in muscle and adipose tissue, relevant primary cells, cell lines, or tissue homogenates from these sources may serve as positive controls for antibody validation.
• To confirm specificity, use appropriate isotype controls and, if available, FATP1 knockout or siRNA knockdown cell models. Perform titration experiments to determine the optimal antibody concentration for your flow cytometry assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering a comprehensive portfolio including validated antibodies for lipid metabolism targets such as FATP1. In addition to this -conjugated monoclonal antibody, we supply bulk ancillary reagents and specialized sourcing for rare targets, bridging the gap from concept to clinic. Our team supports diagnostic manufacturers and research laboratories with streamlined access to critical raw materials and technical expertise, accelerating your assay development and biomarker research.