Anti-Human EG-VEGF/PK1 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P58294

Core Product Specifications and Parameters

Biological Background: EG-VEGF/PK1 (Prokineticin-1) Function and Localization

• Prokineticin-1 (also known as EG-VEGF or mambakine) is a secreted protein that functions as a growth factor and mitogen, encoded by the PROK1 gene.
• Potently contracts gastrointestinal smooth muscle and induces proliferation, migration, and fenestration specifically in capillary endothelial cells derived from endocrine glands, with little effect on other endothelial or non-endothelial cell types.
• Drives proliferation and differentiation (but not migration) of enteric neural crest cells, and directly promotes neuroblastoma cell proliferation and migration.
• Positively regulates PTGS2 expression and prostaglandin synthesis, linking it to inflammatory pathways.
• Implicated in physiological and pathological angiogenesis, including roles in placentation and testis angiogenesis.
• Localizes to glandular epithelium, stroma, and vascular epithelial cells of first trimester decidua at the protein level; expression is upregulated in decidua compared with non-pregnant endometrium.
• Expressed in steroidogenic tissues such as ovary, testis, adrenal gland, and placenta.
• Keywords associated with this target include angiogenesis, disulfide bond, direct protein sequencing, growth factor, mitogen, and secreted signal peptide.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length PROK1 protein (amino acids 1-105), providing broad epitope recognition suitable for applications like WB and ELISA.
• For Western blot, consider using a high-percentage gel (e.g., 15%) to resolve the 12 kDa protein band clearly; a low molecular weight marker is recommended.
• Cross-reactivity with mouse and rat has been reported by the supplier; validate this reactivity in your specific sample type and assay conditions.
• For immunofluorescence (IF-P) and ELISA, optimization of antibody dilution, blocking conditions, and detection system is necessary to obtain robust signals.
• Ensure proper storage and handling; the antibody is shipped on ice bags to maintain activity upon arrival.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD raw materials, offering diagnostic manufacturers and researchers streamlined access to validated antibodies, antigen pairs, and ancillary reagents. This anti-PROK1 monoclonal antibody supports angiogenesis and reproductive biology research, and we can assist with bulk orders, custom formulations, and rare-target sourcing. From concept to clinic, rely on CamelBio to simplify your raw-material supply chain.