Anti-Human E-Cadherin/CD324 Monoclonal Antibody for Flow Cytometry - P12830

Core Product Specifications and Parameters

Biological Background: E-Cadherin Function and Localization

• Encoded by the CDH1 gene, cadherin-1 (E-cadherin) is a calcium-dependent cell adhesion protein that mediates homophilic cell-cell interactions, contributing to the sorting of heterogeneous cell types and the maintenance of epithelial integrity. Related references: PMID:11976333
• It organizes radial actin fiber structures and modulates cellular responses to contractile forces via its interaction with AMOTL2, thereby influencing epithelial mobility and proliferation.
• E-cadherin facilitates the early stages of desmosome assembly by recruiting DSG2 and DSP to cell-cell junctions, coupling adherens junctions with desmosomal complexes. Related references: PMID:29999492
• The protein is predominantly localized at the cell membrane, particularly at adherens junctions and desmosomes, but can also be found in the cytoplasm, endosomes, and the trans-Golgi network. A secreted form has been identified as well.
• Alternative splicing, proteolytic cleavage, and post-translational modifications such as phosphorylation and ubiquitination generate multiple isoforms and functional fragments with distinct roles in signaling and degradation.
• E-cadherin acts as a potent invasion suppressor in epithelial cells and serves as a ligand for integrin αE/β7 on intraepithelial lymphocytes.
• In amyloid metabolism, the C-terminal fragment (E-Cad/CTF2) promotes non-amyloidogenic degradation of APP, reducing the production of neurotoxic Aβ peptides.
• Pathologically, E-cadherin is hijacked as a receptor for Listeria monocytogenes internalin A, promoting bacterial uptake into host cells.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 155–707), ensuring recognition of surface-exposed epitopes suitable for live-cell flow cytometry applications.
• For flow cytometry (FC), titrate the antibody to determine the optimal staining concentration in your specific cell system. Include appropriate isotype controls and confirm specificity using E-cadherin knockout or knockdown models if available.
• Consider that E-cadherin is readily cleaved or shed; surface expression can be influenced by culture conditions, cell density, and fixation/permeabilization protocols. Validate detection parameters under conditions relevant to your experimental model.
• Because E-cadherin forms calcium-dependent homodimers, ensure staining buffers contain physiological calcium concentrations to preserve native conformation and epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This E-cadherin product supports researchers exploring epithelial cell biology, cancer metastasis, and cell adhesion. From concept to clinic, CamelBio serves as your trustworthy procurement partner for high-quality immunoreagents.