Anti-Human DLL4 Monoclonal Antibody () for Flow Cytometry - Q9NR61

Core Product Specifications and Parameters

Biological Background: DLL4 Function and Localization

• Delta-like protein 4 (DLL4), also known as Delta4, AOS6, or hdelta2, is a Notch ligand that activates NOTCH1 and NOTCH4, initiating the Notch signaling pathway. Related references: PMID:11134954
• DLL4 negatively regulates endothelial cell proliferation, migration, and angiogenic sprouting, playing a critical role in angiogenesis. Related references: PMID:20616313
• During spinal cord neurogenesis, DLL4 inhibits V2a interneuron fate, contributing to neuronal subtype specification. Related references: PMID:17728344
• DLL4 is essential for retinal progenitor proliferation; it suppresses rod fates in late retinal progenitors and is required for proper generation of other retinal cell types.
• DLL4 is a single-pass type I membrane protein localized at the cell membrane and is predominantly expressed in vascular endothelium.
• The protein contains multiple EGF-like domains, a transmembrane helix, and disulfide bonds, with a glycosylated molecular weight of approximately 75 kDa (unmodified mass ~74.6 kDa).
• Keywords associated with DLL4 include developmental protein, differentiation, neurogenesis, angiogenesis, sensory transduction, and vision, highlighting its multifaceted roles in development and disease.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 27-524), which includes the EGF repeat region; this design likely provides excellent accessibility to the native receptor on live cells for flow cytometry.
• For flow cytometry, use a directly conjugated antibody; ensure appropriate isotype controls and consider blocking Fc receptors if working with myeloid or endothelial cells.
• The antibody is raised against human DLL4; cross-reactivity with other species has not been determined, so validation in non-human systems is advisable.
• Given the vascular endothelial expression, HUVECs or other endothelial cell lines are suitable positive controls; fixation and permeabilization may be required for intracellular staining protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this DLL4 antibody. We offer one-stop access to bulk ancillary reagents and specialize in sourcing rare targets for Notch signaling, angiogenesis, and developmental research. Partner with CamelBio to streamline your supply chain from concept to clinic.