ABflo 594 Anti-Human Cytokeratin 19 (KRT19) Monoclonal Antibody for Intracellular Flow Cytometry - P08727

Core Product Specifications and Parameters

Biological Background: Cytokeratin 19 (KRT19) Function and Localization

• KRT19 encodes a type I keratin intermediate filament protein, known as Cytokeratin 19 (CK-19) or Keratin 19 (K19).
• In striated muscle, CK-19 partners with KRT8 to anchor the contractile apparatus to the cell membrane via dystrophin at costameres, contributing to myofiber organization.
• Broadly expressed in simple and glandular epithelia: basal keratinocytes of hair follicles, sweat and mammary gland ductal/secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, and ectocervical epithelium.
• Detected in vascular smooth muscle cells of umbilical cord veins and arteries, and in muscle fibers where it localizes to costameres with dystrophin and spectrin.
• Undergoes post-translational modifications such as phosphorylation and methylation, which may regulate filament dynamics and protein interactions.
• Belongs to the intermediate filament family; its coiled-coil domain mediates dimerization essential for filament assembly.
• Involved in host-virus interactions (as annotated by UniProt), although specific mechanisms are not detailed here.
• The protein has a molecular weight of ~44 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 301-400 of human CK-19; the antibody likely recognizes a linear epitope in this region.
• For intracellular flow cytometry, optimize permeabilization and fixation protocols for your specific cell type to ensure epitope accessibility.
• Include appropriate isotype controls and titrate the antibody to determine the optimal staining concentration for your experimental system.
• Validate the antibody’s reactivity in your intended sample type, as fixation and processing conditions can affect epitope availability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering a broad portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This Cytokeratin 19 (KRT19) antibody supports research in epithelial biology, muscle physiology, and related disease models. Our supply network can accommodate bulk orders, rare-target sourcing, and customized development projects, streamlining your path from concept to clinic.