Anti-Human Cytokeratin 18 Monoclonal Antibody for Intracellular Flow Cytometry - P05783

Core Product Specifications and Parameters

Biological Background: Cytokeratin 18 Function and Localization

• Keratin 18 (K18/CK-18/CYK18) is a type I intermediate filament protein encoded by the KRT18 gene, forming obligate heteropolymers with type II keratin KRT8 to provide mechanical stability and resilience to simple epithelial cells. Related references: PMID:15529338, PMID:26823019
• KRT8/KRT18 filaments mediate ARHGEF40-induced actin stress fiber formation and reinforcement under tensional force, and are part of a positive feedback loop downstream of ROCK kinase activation in response to mechanical stress. Related references: PMID:26823019
• Phosphorylation of K18 regulates filament reorganization and is critical for mitotic progression, cellular stress responses, and intermediate filament dynamics. Related references: PMID:7523419, PMID:8522591, PMID:9298992
• K18 participates in the delivery of mutated CFTR to the plasma membrane, influencing cystic fibrosis pathology, and contributes to interleukin-6 (IL-6)-mediated epithelial barrier protection. Related references: PMID:15529338, PMID:16424149, PMID:17213200
• The protein localizes to the nucleus matrix, cytoplasm (perinuclear region), and nucleolus, reflecting its dynamic role in cellular architecture.
• It is expressed in colon, placenta, liver, and weakly in exocervix; expression is upregulated in lymph nodes of breast carcinoma, suggesting a role in cancer progression.
• Post‑translational modifications include acetylation, methylation, ubiquitination, and phosphorylation, which modulate its function and interactions.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, ensure adequate permeabilization (e.g., using saponin or Triton X‑100‑based buffers) to allow antibody access to cytoplasmic intermediate filaments.
• The immunogen spans amino acids 241–430 of human K18; thus, the antibody likely recognizes a linear epitope within this region. Validate reactivity with full‑length and potential caspase‑cleaved fragments in your experimental system.
• As a rabbit monoclonal antibody, it offers high specificity and lot‑to‑lot consistency. Titrate the antibody from 0.1–1 µg per million cells to determine the optimal staining concentration.
• This product is pre‑conjugated to (equivalent to Alexa Fluor® 488). Protect from light and use a 488 nm laser with a 530/30 bandpass filter for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including this anti‑cytokeratin 18 monoclonal antibody for epithelial cell biology and cancer‑related studies. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare target reagents. Partner with CamelBio to streamline your assay development from concept to clinic.