Anti-Human CLEC12A/CD371 Monoclonal Antibody (ABflo 488) for Flow Cytometry - Q5QGZ9-2

Core Product Specifications and Parameters

Biological Background: CLEC12A/CD371 Function and Localization

• CLEC12A (C-type lectin domain family 12 member A) is a C-type lectin receptor, with multiple transcript variants encoding distinct isoforms. This antibody targets isoform 2 (UniProt: Q5QGZ9-2).
• Alternative names include C-type lectin-like molecule 1 (CLL-1), myeloid inhibitory C-type lectin-like receptor (MICL), dendritic cell-associated lectin 2 (DCAL-2), and killer cell C-type lectin-like receptor L1 (hKLRL1), reflecting its association with myeloid lineage cells.
• The gene CLEC12A (GeneID: 160364) is located on human chromosome 12 and encodes proteins involved in immune regulation and cell surface recognition.
• Isoform 2 has a predicted molecular weight of approximately 30.8 kDa. Additional isoforms have predicted masses of 8, 24, 27, 30, and 32 kDa, likely resulting from alternative splicing.
• Flow cytometry application indicates cell surface expression of CLEC12A isoform 2 on target human immune cells.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to ABflo 488, a fluorophore with excitation/emission peaks at 491/516 nm, suitable for single-color or multicolor flow cytometry.
• Titration is recommended to determine the optimal antibody concentration for specific cell populations; use human peripheral blood mononuclear cells or cell lines expressing CLEC12A as positive controls.
• Fc receptor blocking and appropriate isotype controls should be included to minimize non-specific binding.
• Due to the human specificity, cross-reactivity with other species is unlikely; confirm in the relevant sample system if needed.
• For intracellular staining, a fixation/permeabilization protocol may be required as the target is surface-expressed but the immunogen may also detect internal epitopes.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio partners with diagnostic manufacturers and research institutes to deliver a comprehensive range of IVD raw materials, including validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents. Our portfolio spans immune cell surface markers like CLEC12A/CD371, supporting leukemia stem cell research and immunophenotyping. We offer flexible sourcing options from concept to clinic, ensuring you get the right materials for your assay development and production needs.