Anti-Human CEACAM6 Monoclonal Antibody (Conjugate) for Flow Cytometry - P40199

Core Product Specifications and Parameters

Biological Background: CEACAM6 Function and Localization

• CEACAM6 (Carcinoembryonic antigen-related cell adhesion molecule 6), also known as NCA, CEAL, or CD66c, is a cell surface glycoprotein that mediates calcium- and fibronectin-independent homophilic and heterophilic cell adhesion with other CEACAM family members such as CEACAM5 and CEACAM8. Related references: PMID:16204051 PMID:2022629 PMID:11590190
• It plays a significant role in tumor progression and cell migration, facilitating adhesion to endothelial cells. Related references: PMID:16204051
• Heterophilic interaction with CEACAM8 occurs in activated neutrophils, contributing to neutrophil adhesion to cytokine-activated endothelial cells. Related references: PMID:8776764 PMID:1378450
• CEACAM6 is expressed on the apical cell membrane of columnar epithelial and goblet cells of the colon, as well as in neutrophils and numerous tumor cell lines. Related references: PMID:1378450 PMID:10436421 PMID:16204051
• The protein contains immunoglobulin-like domains and is anchored to the membrane via a GPI anchor.
• Post-translational modifications include N-linked glycosylation, and it contains disulfide bonds essential for its structure.
• CEACAM6 is implicated in apoptosis inhibition and is considered an oncogene.

Experimental Guidance and Technical Tips

• For flow cytometry applications, titrate the antibody to determine the optimal working concentration for your specific cell type and experimental conditions.
• Validate the antibody in your cell system of interest, taking into account the reported expression of CEACAM6 in neutrophils, colon epithelial cells, and tumor cell lines.
• Because CEACAM6 is a GPI-anchored protein, ensure that fixation and permeabilization methods are compatible with surface antigen detection if using for intracellular staining.
• Consider using appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding in flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material procurement, offering validated antibody pairs, bulk ancillary reagents, and custom monoclonal antibody development. This anti-CEACAM6 antibody supports oncology and inflammation research, and we can source complementary materials such as antigen standards and blocking buffers. With end-to-end supply chain support, CamelBio accelerates your diagnostic assay development.