Anti-Human CDCP1 Monoclonal Antibody for Flow Cytometry - Q9H5V8

Core Product Specifications and Parameters

Biological Background: CDCP1 Function and Localization

• CDCP1 (CUB domain-containing protein 1), also known as CD318, TRASK, and SIMA135, is a transmembrane glycoprotein and a member of the tetraspanin web involved in tumor progression and metastasis.
• It may mediate cell adhesion and cell matrix association, contributing to the regulation of anchorage, migration, proliferation, and differentiation through its phosphorylation status.
• CDCP1 has been proposed as a novel marker for leukemia diagnosis and for identifying immature hematopoietic stem cell subsets.
• The protein localizes to the cell membrane and can be secreted; alternative splicing produces multiple isoforms.
• It is highly expressed in mitotic cells, with highest levels in skeletal muscle and colon, and lower expression in kidney, small intestine, placenta, and lung.
• CDCP1 is upregulated in various human cancers, including colorectal cancer, breast carcinoma, and lung cancer, and is expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 30–666 of human CDCP1, corresponding to the extracellular region, suggesting this antibody likely recognizes an extracellular epitope suitable for surface staining in flow cytometry without permeabilization.
• For flow cytometry, titrate the antibody to determine optimal concentration and use appropriate isotype controls. Surface staining of live cells is recommended; if intracellular staining is needed, validate fixation and permeabilization conditions.
• The target protein has observed molecular weights of 37, 72, and 92 kDa, possibly due to isoforms or differential glycosylation. If employing this antibody for Western blotting, confirm specificity by observing the expected band pattern in positive control lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—from concept to clinic. This anti-human CDCP1 monoclonal antibody supports research in cancer biology, stem cell studies, and diagnostic assay development. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your procurement and accelerate assay design.