Anti-Human CD99 Monoclonal Antibody () for IF/ICC, FC - P14209

Core Product Specifications and Parameters

Biological Background: CD99 Antigen Function and Localization

• CD99, also known as MIC2, E2 antigen, or T-cell surface glycoprotein E2, is a transmembrane glycoprotein encoded by the CD99 gene (GeneID: 4267).
• It mediates T-cell adhesion and facilitates spontaneous rosette formation with erythrocytes (By similarity).
• During leukocyte extravasation, CD99 acts independently of PECAM1 to help cells traverse the endothelial basement membrane (By similarity).
• Localized to the plasma membrane as a single-pass type I membrane protein.
• Alternative splicing generates multiple isoforms.
• Post-translational modifications include N-linked glycosylation and phosphorylation.
• Keywords: 3D-structure, Alternative splicing, Cell adhesion, Glycoprotein, Membrane, Phosphoprotein, Signal, Transmembrane helix.

Experimental Guidance and Technical Tips

• This antibody is supplied as a direct conjugate to , eliminating the need for a secondary antibody in IF/ICC and FC applications.
• The immunogen corresponds to amino acids 24-123 of human CD99; verify that your target epitope is present in the sample or isoform under study.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your cell type and experimental conditions.
• In immunofluorescence, include appropriate controls (e.g., isotype control) and follow standard fixation/permeabilization protocols.
• CD99 is expressed on the cell surface; live-cell staining is feasible if the epitope is extracellular.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-human CD99 monoclonal antibody is ideal for T-cell adhesion and leukocyte trafficking studies. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development.