Anti-Human CD98 Monoclonal Antibody () for Flow Cytometry - P08195

Core Product Specifications and Parameters

Biological Background: CD98 (SLC3A2) Function and Localization

• The CD98 heavy chain (SLC3A2) functions as a chaperone that facilitates biogenesis and trafficking of SLC7 family amino acid transporter heterodimers to the plasma membrane. Related references: PMID:10574970, PMID:10903140, PMID:11557028

• Heterodimers of CD98 with SLC7A6 or SLC7A7 mediate uptake of dibasic amino acids, while the SLC3A2/SLC7A11 antiporter exchanges extracellular cystine for intracellular glutamate. Related references: PMID:10903140, PMID:9829974, PMID:34880232

• CD98 is essential for the plasma membrane localization, stability, and transport activity of SLC7A5 and SLC7A8, which translocate neutral amino acids, thyroid hormones, and L-DOPA. Related references: PMID:10391915, PMID:10574970, PMID:11311135, PMID:11389679, PMID:11557028

• In lysosomes, the SLC3A2-SLC7A5 complex, when associated with LAPTM4B, promotes leucine uptake and activates mTORC1 signaling. Related references: PMID:25998567

• CD98 modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration, and tumor progression. Related references: PMID:11121428, PMID:15625115

• Ubiquitously expressed with highest levels in kidney, placenta, and testis; also detected in endothelial cells, lymphocytes, and fetal liver.

• Acts as a receptor for Plasmodium vivax in reticulocytes and facilitates hepatitis C virus entry via SLC3A2/SLC7A5.

• Post-translational modifications include N-glycosylation, disulfide bonds, and phosphorylation; the protein is a transmembrane glycoprotein with a type II topology.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to (excitation ~488 nm), suitable for flow cytometry without secondary staining; titrate to determine optimal staining concentration.
• Include an appropriate isotype control and single-color compensation controls to ensure accurate gating in multicolor panels.
• Validate staining in known CD98-positive cell lines (e.g., HeLa, Jurkat, HEK293) and use a CD98-negative line or Fc-blocking reagent to confirm specificity.
• The immunogen (aa 206–630) covers most of the extracellular domain, so the antibody is expected to detect surface CD98; fixation and permeabilization may alter epitope accessibility.
• For sample preparation, collect live cells and stain immediately; avoid prolonged fixation before staining if possible.

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