Anti-Human CD96/TACTILE Monoclonal Antibody for Flow Cytometry - P40200

Core Product Specifications and Parameters

Biological Background: T-cell surface protein tactile Function and Localization

• CD96 (TACTILE) is a T-cell surface protein belonging to the immunoglobulin superfamily, encoded by the CD96 gene; alternative splicing produces multiple isoforms.
• It promotes adhesive interactions of activated T and NK cells during the late phase of the immune response, enhancing NK cell-target adhesion by binding to the poliovirus receptor (PVR/CD155) on target cells.
• CD96 is expressed on normal T-cell lines and clones, some transformed T-cells, and at very low levels on activated B-cells, but not on other cultured cell lines tested.
• The protein localizes to the plasma membrane as a type I transmembrane protein, with an extracellular region containing immunoglobulin-like domains, a single transmembrane helix, and a cytoplasmic tail.
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, contributing to structural stability and ligand interaction.
• CD96 is associated with chromosomal rearrangements and has been implicated in craniosynostosis, with disease-associated variants reported.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry (FC); consider titrating from 0.1–1 µg per million cells in a suitable staining buffer.
• Include appropriate isotype controls and live/dead discrimination to ensure accurate gating.
• The immunogen encompasses amino acids 25–519, covering most of the extracellular domain; the antibody likely recognizes an epitope within this region.
• Validate staining on relevant T-cell or NK-cell lines (e.g., Jurkat, NKL) or primary human peripheral blood mononuclear cells, as CD96 expression is largely restricted to activated T and NK cells.
• For dual-color FC, confirm that the conjugate does not exhibit unexpected spectral interference with other fluorophores in your panel.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents — all under one roof. This anti-CD96/TACTILE antibody exemplifies our commitment to providing critical tools for immune-cell adhesion and activation research. Whether you need rare-target antibodies, recombinant proteins, or custom sourcing for diagnostic development, CamelBio bridges the gap from concept to clinic with responsive technical support and reliable supply chains.