Anti-Human CD94 Monoclonal Antibody for FC - Q13241

Core Product Specifications and Parameters

Biological Background: CD94 Function and Localization

• CD94 (KLRD1) is a C-type lectin transmembrane receptor expressed as a disulfide-linked dimer, serving as a ligand-binding subunit for NK cell receptors KLRC1 and KLRC2.
• CD94-KLRC1 and CD94-KLRC2 heterodimers recognize non-classical MHC class I molecule HLA-E presenting self-peptides, enabling immune surveillance of MHC class I expression. Related references: PMID:10023772 PMID:18064301 PMID:18083576
• The CD94-KLRC1 inhibitory receptor counteracts activating signals via ITIM-mediated recruitment of tyrosine phosphatases, crucial for self-tolerance and tumor immune evasion. Related references: PMID:12165520 PMID:30860984 PMID:9430220 PMID:9485206 PMID:30503213
• The CD94-KLRC2 activating receptor triggers cell activation through the adaptor DAP12, contributing to adaptive NK cell responses and maternal-fetal tolerance. Related references: PMID:15940674 PMID:20952657 PMID:21825173 PMID:30134159 PMID:9655483 PMID:9754572
• CD94 is predominantly expressed on the cell membrane of NK cells and subsets of CD8+ αβ and γδ T cells, with distinct expression patterns of inhibitory and activating complexes. Related references: PMID:12387742 PMID:20952657 PMID:21825173 PMID:9430220 PMID:9485206
• Viral pathogens such as SARS-CoV-2 exploit CD94-mediated HLA-E recognition to induce NK cell exhaustion and dampen antiviral immunity. Related references: PMID:32859121

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 32-179 of human CD94, which covers the extracellular C-type lectin-like domain. This region is likely to generate antibodies recognizing native CD94 on live cells, making it suitable for flow cytometry.
• As this antibody is validated for flow cytometry (FC), consider using it for surface staining of NK cells or T cell subsets. Include appropriate isotype controls and optimize antibody titration for your specific experimental setup.
• Cross-reactivity is reported for human; if working with other species, validate the antibody in the relevant sample system to confirm reactivity.
• The molecular weight of CD94 is approximately 21 kDa. While this antibody is recommended for FC, the known molecular weight may be useful if you attempt complementary applications such as western blotting, but suitability for other applications should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with comprehensive access to IVD raw materials, supporting every stage from concept to clinic. This anti-CD94 monoclonal antibody is sourced to aid research in natural killer cell biology and immune checkpoint studies. Our offerings include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom rare-target material sourcing to accelerate your assay development.