Anti-Human CD90/Thy1 Rabbit Monoclonal Antibody for Flow Cytometry - P04216

Core Product Specifications and Parameters

Biological Background: CD90/Thy1 Function and Localization

• CD90/Thy1 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein belonging to the immunoglobulin superfamily.
• The protein is a single-pass type I membrane protein located on the outer leaflet of the cell membrane.
• It is also known as Thy-1 antigen or CDw90, encoded by the THY1 gene in humans.
• CD90/Thy1 may participate in cell-cell and cell-ligand interactions, particularly during synaptogenesis and other neural events.
• The molecule contains a single immunoglobulin-like domain in its extracellular region.
• Key structural features include a disulfide bond, N-linked glycosylation, and a GPI anchor that tethers it to the membrane.
• Post-translational modifications include phosphorylation and pyrrolidone carboxylic acid formation at the N-terminus.
• Expression is not restricted to the brain; CD90 is found on various cell types including hematopoietic stem cells, neurons, and subsets of fibroblasts.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody comprises amino acids 20-130 of the extracellular domain, which is predicted to be highly immunogenic and surface-accessible.
• For flow cytometry, directly conjugated allows single-step staining of live cells; consider titrating the antibody to determine optimal staining concentration.
• Since CD90 is a GPI-anchored protein, ensure that cell preparation and staining conditions preserve membrane integrity to avoid loss of signal.
• This antibody is validated for human reactivity; verify cross-reactivity in your species of interest if needed.
• Include appropriate positive and negative controls (e.g., CD90-expressing cell lines vs. knockout or irrelevant cell types) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a wide array of IVD raw materials including this anti-CD90 antibody, supporting research into cell surface markers and neuroimmunology. Whether you need validated antibody pairs, bulk monoclonal/polyclonal antibodies, or ancillary reagents, our one-stop platform bridges the gap from early development to clinical application. For hard-to-source targets, our network delivers customized solutions to accelerate your diagnostic project.