Anti-Human CD8b Monoclonal Antibody () for Flow Cytometry - P10966

Core Product Specifications and Parameters

Biological Background: CD8b Function and Localization

• CD8b is an integral membrane glycoprotein that serves as a co-receptor for MHC class I molecules, playing an essential role in the adaptive immune response.
• It forms a heterodimer with CD8a and interacts with the TCR-CD3 complex to recognize peptide-MHC class I complexes on antigen-presenting cells.
• Upon antigen recognition, CD8b-associated LCK kinase initiates intracellular signaling cascades that promote T-cell activation, cytokine production, cytotoxicity, and proliferation.
• The cytoplasmic tail contains a palmitoylated cysteine that anchors the CD8 co-receptor in lipid rafts, facilitating efficient signal transduction.
• Alternative splicing generates multiple isoforms (isoforms 1, 3, 5, 6, 7, 8) that are differentially expressed in the thymus and peripheral CD8+ T-cells, suggesting specialized roles in T-cell development and peripheral function.
• Subcellularly, CD8b is localized to the cell membrane and may also be found in secreted forms.
• Keywords: adaptive immunity, T-cell co-receptor, immunoglobulin domain, transmembrane glycoprotein, phosphoprotein, lipoprotein.

Experimental Guidance and Technical Tips

• For flow cytometry, we recommend titrating the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• The recombinant protein immunogen spanning the extracellular region (aa 22-170) suggests good recognition of cell surface CD8b; validate in relevant human sample systems (e.g., PBMCs, lymphoid tissues).
• Ensure minimal light exposure to preserve the fluorophore; use appropriate compensation controls in multicolor panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-human CD8b antibody supports immune monitoring, immuno-oncology, and T-cell research applications. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—streamlining your procurement and accelerating development.