Anti-Human CD82 Monoclonal Antibody for FC - P27701

Core Product Specifications and Parameters

Biological Background: CD82 Function and Localization

• CD82, also known as KAI1, C33, IA4, R2, and TSPAN27, is a member of the tetraspanin superfamily and functions as a metastasis suppressor.
• It is a structural component of tetraspanin-enriched microdomains (TERMs), which serve as platforms for receptor clustering and signaling. Related references: PMID:19497983
• Attenuates EGF signaling by facilitating EGFR endocytosis and desensitization, and regulates EGFR ubiquitination via CBL phosphorylation. Related references: PMID:10985391 PMID:35538033 PMID:23897813
• Modulates integrin-mediated cell adhesion and migration, including regulation of integrins alpha4, alpha6, and beta1. Related references: PMID:24623721 PMID:8757325 PMID:15557282 PMID:17560548
• Interferes with plasminogen activation by disrupting the PLAU-PLAUR interaction. Related references: PMID:15677461
• Plays roles in immune responses: costimulation of TCR/CD3 pathway, TLR9 trafficking (By similarity), and inhibition of LPS-induced TLR4 signaling. Related references: PMID:36945827
• Controls angiogenesis by binding and sequestering VEGFA and PDGFB, switching the angiogenic milieu to a quiescent state. Related references: PMID:34530889
• Subcellular localization: cell membrane, cytoplasmic vesicle, phagosome. Expressed in lymphoid tissues.

Experimental Guidance and Technical Tips

• For flow cytometry, consider titrating the antibody to determine the optimal staining concentration for your specific cell system.
• The immunogen corresponds to amino acids 1–267 of human CD82; verify that your target epitope is within this region, as some splice variants may not be recognized.
• Confirm cross-reactivity and specificity in your experimental setting, as this antibody is validated for human samples.
• Include appropriate isotype controls and fluorescence compensation when using this conjugated antibody in multicolor panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-CD82 reagent, optimized for applications such as flow cytometry. Whether you need antibody pairs, bulk ancillary reagents, or rare target sourcing for tetraspanin and metastasis research, we provide one-stop access from concept to clinic. Partner with us to streamline your diagnostic development with reliable, high-quality raw materials and expert technical support.