Anti-Human CD79a Monoclonal Antibody for Intracellular Flow Cytometry - P11912

Core Product Specifications and Parameters

Biological Background: CD79a Function and Localization

• CD79a, also known as Ig-alpha or MB-1, is a single-pass type I membrane protein that serves as the alpha chain of the B-cell antigen receptor complex (BCR).
• It is essential for BCR surface expression and for the initiation of signal transduction upon antigen binding, leading to B-cell activation, internalization, and antigen presentation.
• In cooperation with CD79b, CD79a stimulates SYK kinase autophosphorylation and recruits BLNK, facilitating the phosphorylation cascade required for B-cell development and function.
• During B-cell ontogeny, CD79a plays a critical role in efficient differentiation of pro- and pre-B-cells, while also modulating BCR signaling to repress activation in immature B cells.
• Subcellularly, CD79a is primarily localized to the cell membrane, consistent with its role as a transmembrane receptor component.
• Expression is highly tissue-specific, restricted to B-cells, making it a reliable pan-B-cell marker for immunological studies and diagnostics.
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, which regulate its stability and signaling activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 165–225 of human CD79a (intracellular domain), which is appropriate for intracellular detection methods such as intracellular flow cytometry.
• As an conjugate, this antibody enables direct detection; consider optimizing fixation and permeabilization protocols for your specific cell type to maintain epitope accessibility.
• Validate antibody performance in relevant human B-cell lines (e.g., Raji, Daudi) or primary peripheral blood mononuclear cells (PBMCs) to confirm specificity and sensitivity.
• CD79a staining intensity may vary with B-cell maturation stage; correlate with other B-cell markers (e.g., CD19, CD20) to accurately profile B-cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and laboratories. This anti-human CD79a monoclonal antibody supports B-cell immunology and hematological research applications. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your project from concept to clinic.