Anti-Human CD79a Monoclonal Antibody for Flow Cytometry (Intracellular) - P11912

Core Product Specifications and Parameters

Biological Background: CD79a Function and Localization

• CD79a, also known as Ig-alpha or MB-1, is the alpha chain of the B-cell antigen receptor complex and contains an immunoglobulin domain.
• In cooperation with CD79B, it is essential for initiating signal transduction following antigen binding to the B-cell receptor (BCR), leading to BCR internalization, trafficking to late endosomes, and antigen presentation.
• CD79a is required for BCR surface expression and efficient differentiation of pro- and pre-B-cells.
• It stimulates SYK autophosphorylation and activation, and recruits BLNK to the receptor complex, facilitating BLNK phosphorylation by SYK.
• CD79a interacts with and enhances the activity of some Src-family tyrosine kinases, while also repressing BCR signaling during the development of immature B-cells.
• Subcellular localization is at the cell membrane, consistent with its role as a transmembrane receptor component.
• The protein is specifically expressed in B-cells and undergoes post-translational modifications including glycosylation, disulfide bond formation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular domain (amino acids 165–225) of human CD79a, making this antibody suitable for intracellular flow cytometry after permeabilization.
• For flow cytometry, consider titrating the antibody and using appropriate permeabilization buffers validated for nuclear/membrane proteins.
• The antibody is specific for human CD79a; cross-reactivity with other species has not been tested.
• Given the role of CD79a in B-cell receptor signaling, this antibody can be used to study B-cell development, activation, and lymphomas.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents for immunoassay development. For researchers studying B-cell biology and hematological malignancies, we provide ready-to-use monoclonal antibodies against CD79a and other lymphocyte markers, along with bulk supply and custom sourcing options. Our integrated supply chain supports every stage from concept to clinic, ensuring consistent quality and reliable delivery for diagnostic manufacturers and research laboratories.