Anti-Human CD72 Monoclonal Antibody () for Flow Cytometry - P21854

Core Product Specifications and Parameters

Biological Background: CD72 Function and Localization

• CD72, also known as Lyb-2, is a B-cell differentiation antigen and a type II transmembrane glycoprotein.
• Acts as a co-receptor of the B cell receptor (BCR), playing both positive and negative regulatory roles in B-cell functions.
• Negative signaling: recognizes the Sm/ribonucleoprotein (RNP) self-antigen; coligation with BCR recruits PTPN6/SHP-1, inhibiting BCR signaling.
• Serves as a ligand for CD5, playing a critical role in maintaining regulatory T and B-cell homeostasis.
• Subcellular localization: membrane-bound; extracellular domain mediates ligand interaction.
• Tissue specificity: expressed in pre-B-cells and B-cells, but absent from terminally differentiated plasma cells.
• Post-translational modifications include disulfide bonds, glycosylation, and phosphorylation, consistent with its receptor and signaling functions.
• Contains a C-type lectin domain and a signal-anchor transmembrane helix, classifying it as a receptor with lectin activity.

Experimental Guidance and Technical Tips

• The immunogen (aa 117-359) corresponds to the extracellular domain, suggesting the antibody likely targets the surface-expressed CD72; validate in your specific cell system.
• Optimize flow cytometry staining by titrating the antibody concentration to achieve the best signal-to-noise ratio for human B cells.
• Include appropriate controls (e.g., isotype control, Fc block) to account for non-specific binding, especially in primary B-cell samples.
• Confirm expression in your target cell type, as CD72 is not present on plasma cells; pre-B and mature B cells are positive.
• This antibody has been validated for flow cytometry; for other applications, additional verification is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with seamless access to IVD raw materials, from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing, all backed by technical consulting. Explore our CD72 and B-cell marker offerings to accelerate your immunoassay development with reliable, high-quality reagents.