Anti-Human CD70 (CD27L) Monoclonal Antibody for Flow Cytometry - P32970

Core Product Specifications and Parameters

Biological Background: CD70 Function and Localization

• CD70, also known as CD27 ligand (CD27L) or TNFSF7, is a type II transmembrane protein and member of the tumor necrosis factor (TNF) ligand family; alternative splicing generates multiple isoforms.
• Expressed at the plasma membrane of B cells, CD70 serves as the ligand for CD27, which is specifically expressed on the surface of T cells. Related references: PMID:28011863, PMID:28011864, PMID:8387892
• The CD70-CD27 interaction triggers antigen-specific T cell activation and clonal expansion, providing critical immune surveillance of B cells. Related references: PMID:28011863, PMID:28011864
• CD70 is a single-pass membrane protein with an extracellular C-terminal TNF homology domain, stabilized by disulfide bonds and glycosylation.
• Subcellularly, CD70 localizes to the cell membrane, consistent with its role in intercellular communication.
• Soluble forms of CD70 can be shed from the cell surface through alternative splicing, potentially modulating immune responses.
• The CD27/CD70 pathway is implicated in lymphocyte costimulation, antiviral immunity, and tumor immunity, positioning CD70 as a target for cancer immunotherapy and autoimmune disease research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 39-193) of human CD70; consider using a protease inhibitor cocktail to prevent shedding during cell preparation for flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and assay conditions, and include an appropriate isotype control.
• As CD70 is expressed on activated immune cells, stimulating primary lymphocytes or using relevant cell lines may enhance detection; validate expression in your sample system prior to quantitative analysis.
• Note that CD70 can be shed from the cell surface; short staining times and ice-cold buffers may help preserve surface labeling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents, to accelerate your diagnostic development. The Anti-Human CD70 antibody supports precise flow cytometric analysis of immune cell interactions with reliable batch-to-batch consistency. From rare target sourcing to custom orders, trust CamelBio as your partner from concept to clinic.