Anti-Human CD7 Monoclonal Antibody for FC - P09564

Core Product Specifications and Parameters

Biological Background: T-cell antigen CD7 Function and Localization

• CD7, also known as GP40, T-cell leukemia antigen, Leu-9, or TP41, is a single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It is expressed on T-cells and natural killer (NK) cells and their precursors. Related references: PMID:7506726
• Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. Related references: PMID:10652336
• Mediates the production of cytokines such as IL-2, contributing to T-cell proliferation and differentiation. Related references: PMID:1709867
• On resting NK cells, CD7 activation results in a significant induction of interferon-gamma levels. Related references: PMID:7506726
• Localized to the cell membrane, with an extracellular immunoglobulin-like domain and a short cytoplasmic tail.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and palmitoylation, which may influence membrane microdomain localization and signaling.

Experimental Guidance and Technical Tips

• The immunogen (aa 26–180) covers the extracellular domain, enabling recognition of native surface CD7.
• The conjugate allows direct detection without a secondary reagent; ensure instrument compatibility with 633/635 nm excitation and APC-A700 emission filters.
• For multicolor panels, titrate the antibody to minimize spillover and optimize resolution; include Fc receptor blocking and viability dye.
• Validate gating strategies using lineage markers (e.g., CD3 for T cells, CD56 for NK cells) and appropriate isotype controls.
• Consider fixation and permeabilization conditions if surface staining is combined with intracellular markers, as CD7 expression can be modulated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive portfolio of IVD raw materials, from target discovery to clinical validation. This anti-human CD7 antibody exemplifies our monoclonal antibody solutions for immunophenotyping and immuno-oncology applications. Explore our catalog for validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare-target raw materials to accelerate your assay development and commercialization.