Anti-Human CD68 Monoclonal Antibody (PerCP) for Flow Cytometry (Intracellular) - P34810

Core Product Specifications and Parameters

Biological Background: Macrosialin (CD68) Function and Localization

• CD68 (macrosialin/gp110) is a heavily glycosylated type I transmembrane protein belonging to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family.
• Plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions.
• Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites; rapid recirculation from endosomes/lysosomes to the plasma membrane may facilitate macrophage crawling.
• Highly expressed by blood monocytes and tissue macrophages; also found in lymphocytes, fibroblasts, endothelial cells, and many tumor cell lines, where it may mediate attachment to vascular endothelium and dissemination.
• Subcellular localization includes cell membrane, endosome membrane, and lysosome membrane, consistent with its role in endosomal/lysosomal trafficking.
• Contains disulfide bonds, is N-glycosylated, and exhibits alternative splicing; its predicted molecular weight is ~37 kDa but appears larger due to glycosylation.
• Key features: alternative splicing, disulfide bond, glycoprotein, signal, transmembrane helix, and repeat domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-319) of human CD68; it may recognize both glycosylated and unglycosylated forms. For intracellular staining, consider fixation and permeabilization protocols optimized for lysosomal markers.
• This PerCP-conjugated antibody is intended for intracellular flow cytometry (FC intra); validate the staining procedure in the relevant sample system (e.g., human PBMCs or tissue macrophages) with appropriate isotype controls.
• CD68 is a classical macrophage marker, but it can be expressed in some non-myeloid cells (e.g., activated endothelial cells, fibroblasts). Confirm specificity in your target cell population.
• When designing multicolor panels, check PerCP's excitation/emission profile and potential spillover from other fluorochromes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated monoclonal/polyclonal antibodies, antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing, supporting macrophage biology, lysosomal storage disorders, and tumor microenvironment research. Contact us to streamline your CD68-related assay development.