Anti-Human CD66b Monoclonal Antibody for Flow Cytometry - P31997

Core Product Specifications and Parameters

Biological Background: CEACAM8 Function and Localization

• CEACAM8 (CD66b) is a glycosylphosphatidylinositol (GPI)-anchored member of the carcinoembryonic antigen (CEA) family, primarily expressed on neutrophils. It is also referred to as CD67, CGM6, and NCA-95.
• The protein mediates calcium-independent, heterophilic cell adhesion, interacting with other CEACAMs like CEACAM6. This interaction is important for neutrophil activation and migration (PubMed:11590190, PubMed:2022629, PubMed:8776764). Related references: PMID:11590190 PMID:2022629 PMID:8776764
• Subcellularly, CEACAM8 localizes to the cell membrane and cell surface via its GPI anchor, positioning it for extracellular interactions.
• It is predominantly expressed in leukocytes from chronic myeloid leukemia patients and in bone marrow, highlighting its role in myeloid cell biology.
• Post-translational modifications include glycosylation and disulfide bond formation, contributing to its structural stability and immune recognition.
• UniProt keywords: 3D-structure, Cell adhesion, Cell membrane, Disulfide bond, Glycoprotein, GPI-anchor, Immunoglobulin domain, Lipoprotein, Membrane, Proteomics identification, Reference proteome, Repeat, Signal.

Experimental Guidance and Technical Tips

• This pre-conjugated antibody is ready for direct flow cytometry, eliminating the need for secondary staining and reducing experimental variability.
• The immunogen (aa 35-319) spans the extracellular domain, suggesting that the antibody likely recognizes epitopes accessible on live cells; consider titrating to determine optimal staining concentrations for your specific sample.
• Include positive controls such as activated neutrophils or CD66b-expressing cell lines to validate performance in your experimental system.
• Protect the conjugate from light and avoid repeated freeze-thaw cycles to maintain fluorescence intensity and antibody stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal antibodies, and ancillary reagents. This anti-CD66b antibody supports neutrophil and leukemia research, aligning with our commitment to supplying high-quality tools for immune-oncology applications. For customized sourcing or bulk requirements, CamelBio offers one-stop technical support from concept to clinic.