Anti-Human CD59 Monoclonal Antibody for Flow Cytometry - P13987

Core Product Specifications and Parameters

Biological Background: CD59 Function and Localization

• CD59, also known as protectin, MAC-inhibitory protein (MAC-IP), 20 kDa homologous restriction factor (HRF-20), and 1F5 antigen, is a GPI-anchored cell surface glycoprotein that functions as a critical inhibitor of the complement membrane attack complex (MAC).
• It binds to the C8 component (C8A and C8B) of the assembling MAC, forming an intermolecular beta-sheet that prevents incorporation of multiple C9 molecules, thereby blocking formation of the osmolytic pore and protecting human cells from complement-mediated lysis (PubMed:11882685, PubMed:1698710, PubMed:2475111, PubMed:36797260). Related references: PMID:11882685, PMID:1698710, PMID:2475111, PMID:36797260
• A soluble form of CD59, found in urine, retains complement binding activity but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
• The protein is localized to the cell membrane via a GPI anchor; a secreted form has also been detected.
• CD59 undergoes alternative splicing and post-translational modifications including disulfide bond formation, glycation, and N-linked glycosylation; mutations in CD59 are associated with hereditary hemolytic anemia.
• The predicted molecular mass of CD59 is 14177 Da, consistent with its observed migration at ~14–18 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (aa 26–101) of human CD59, which includes the key functional sites for MAC binding; this design may favor recognition of native, properly folded conformations.
• For flow cytometry, consider using live, unfixed cells to preserve cell surface GPI-anchored CD59; Fc receptor blocking is recommended to minimize non-specific binding.
• Titrate the antibody under your specific experimental conditions to determine the optimal dilution; protect the conjugate from light and avoid repeated freeze-thaw cycles to maintain fluorophore stability.
• Validate specificity using CD59-positive cells (e.g., leukocytes) and CD59-knockout or deficient controls; complementary detection with an anti-GPI anchor marker can confirm membrane localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to validated IVD raw materials, including monoclonal antibodies like this anti-CD59 clone, supporting complement biology and immune research from concept to clinic. Our one-stop platform supplies diagnostic manufacturers and laboratories with optimized antibody pairs, bulk ancillary reagents, and rare-target raw material sourcing to streamline assay development. For tailored solutions or technical consulting, CamelBio bridges your project from discovery to clinical application.