Anti-Human CD59 Monoclonal Antibody for Flow Cytometry (FC) - P13987

Core Product Specifications and Parameters

Biological Background: CD59 Function and Localization

• CD59, also known as protectin, MAC-inhibitory protein (MAC-IP), and 20 kDa homologous restriction factor (HRF20), is a GPI-anchored glycoprotein that serves as a critical complement regulator.
• It functions as a potent inhibitor of the membrane attack complex (MAC) by binding to C8 and C9 components, preventing formation of the cytolytic pore. Related references: PMID:11882685 PMID:1698710 PMID:2475111 PMID:36797260
• CD59 is widely expressed on human cells, anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) moiety, and protects host cells from autologous complement-mediated lysis.
• A soluble form, found in urine, retains complement binding but exhibits greatly reduced MAC-inhibitory activity compared to the membrane-bound isoform.
• The protein undergoes post-translational modifications including disulfide bond formation, glycation, and N-linked glycosylation; mutations are linked to hereditary hemolytic anemia.
• Alternative splicing generates multiple transcript variants, and the protein has been identified via direct protein sequencing and large-scale proteomics.
• Subcellularly, CD59 localizes to the cell membrane and is also secreted, contributing to immune evasion and signaling beyond complement inhibition.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 26–101 of human CD59, which corresponds to the mature extracellular domain; the antibody is expected to recognize native, membrane-bound CD59. Consider using a matched isotype control for flow cytometry experiments.
• For flow cytometry, titrate the -conjugated antibody on human peripheral blood mononuclear cells (PBMCs) or CD59-expressing cell lines to determine optimal staining concentration.
• CD59 is GPI-anchored; avoid treatments that may release GPI-anchored proteins (e.g., phospholipase C) unless specifically evaluating anchor dependence. Validate in the relevant sample system.
• Cross-reactivity is reported for human only; if working with non-human models, validate specificity and reactivity before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and clinical institutions with streamlined access to a comprehensive range of IVD raw materials. This -conjugated anti-CD59 monoclonal antibody supports complement biology research and flow cytometry-based diagnostics. As a dedicated sourcing partner, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target procurement to accelerate your assay development.