Anti-Human CD56 Monoclonal Antibody for Flow Cytometry - P13591

Core Product Specifications and Parameters

Biological Background: Neural Cell Adhesion Molecule 1 Function and Localization

• NCAM1 (CD56) is a cell adhesion molecule of the immunoglobulin superfamily, mediating homophilic neuron-neuron adhesion and promoting neurite fasciculation and outgrowth.
• The protein functions as a receptor for rabies virus and Zika virus, facilitating viral entry into host cells.
• Multiple protein isoforms are produced by alternative splicing, with observed molecular weights ranging from approximately 40 kDa to 95 kDa.
• Subcellular localization includes the cell membrane via a GPI anchor or transmembrane domain, and it can also be secreted into the extracellular space.
• Key post-translational modifications include N-linked glycosylation, phosphorylation, and disulfide bond formation, as annotated in UniProt.
• The gene NCAM1 is associated with keywords such as cell adhesion, host-virus interaction, and alternative splicing in UniProtKB reviewed records.

Experimental Guidance and Technical Tips

• For flow cytometry, it is advisable to titrate the antibody to determine the optimal dilution for your specific cell type and experimental setup.
• Include an appropriate isotype control to assess non-specific binding and ensure accurate population gating.
• Given the multiple isoforms, confirm that the antibody recognizes your target isoform under your denaturing or native conditions when extending applications beyond FC.
• Store and handle according to standard antibody protocols, avoiding repeated freeze-thaw cycles to maintain functional integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For scientists investigating neural cell adhesion or viral entry mechanisms, we offer consistent access to CD56-related reagents and can source rare-target raw materials to accelerate your development pipeline. Our technical consulting and supply chain solutions support every stage, from early-stage research to clinical applications.