Anti-Human CD55 (DAF) Rabbit Monoclonal Antibody () for Flow Cytometry - P08174

Core Product Specifications and Parameters

Biological Background: CD55 (Complement decay-accelerating factor) Function and Localization

• CD55, also known as decay-accelerating factor (DAF), is a GPI-anchored membrane protein that belongs to the regulators of complement activation (RCA) family.
• It functions as a key negative regulator of the complement cascade by binding to C4b and C3b fragments, thereby preventing the formation and accelerating the decay of C3 and C5 convertases (PubMed:7525274, PubMed:28657829). Related references: PMID:7525274 PMID:28657829
• CD55 is expressed on plasma membranes of all cell types in contact with plasma complement proteins, as well as on epithelial cells and in body fluids.
• The protein is heavily glycosylated and exhibits multiple molecular weight isoforms (39-59 kDa) due to alternative splicing and post-translational modifications.
• In addition to complement regulation, CD55 serves as a receptor for various enteroviruses, including coxsackieviruses and echoviruses.
• Keywords associated with CD55 include GPI-anchor, sushi (SCR) repeats, innate immunity, and host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 35-353 of human CD55) covers the majority of the extracellular domain, including the four sushi (SCR) repeats. This suggests the antibody likely recognizes an epitope in this region, which is important for ligand binding.
• As a monoclonal rabbit antibody conjugated to , it is optimized for direct flow cytometry. For best results, titrate the antibody and include appropriate isotype and compensation controls.
• CD55 is expressed on most cell surfaces; thus, consider using a suitable viability dye and gate on live cells to reduce background from dead cells.
• Due to variable glycosylation, the observed molecular weight of CD55 may differ between cell types. If adapting this antibody for Western blotting, validate the specificity in the target sample.
• Always validate the antibody in your specific experimental system, as performance may vary with sample preparation and fixation protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-CD55 clone, for complement and infectious disease research. In addition to ready-to-use antibodies, we provide custom development of antibody pairs, bulk ancillary reagents, and sourcing services for rare or hard-to-find targets. Partner with us to streamline your diagnostic and research supply chain.