Anti-Human CD5 Monoclonal Antibody for Flow Cytometry - P06127

Core Product Specifications and Parameters

Biological Background: CD5 Function and Localization

• CD5 is a lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells.
• It plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation, and immune tolerance.
• CD5 acts as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K, and CBL. Related references: PMID:1384049 PMID:1385158
• Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. Related references: PMID:23376399
• CD5 modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production. Related references: PMID:27499044
• It interacts with several ligands expressed on B-cells such as CD5L or CD72, playing an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis.
• Subcellular localization is at the cell membrane, consistent with its role as a transmembrane receptor.
• Post-translational modifications include disulfide bonds, glycosylation, and phosphorylation, which are critical for its signaling functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 25-371), which is well-suited for detecting cell surface CD5 in flow cytometry.
• Consider titrating the antibody to determine the optimal dilution for your specific cell type, as staining intensity may vary.
• Validate the antibody in human T-cell lines such as Jurkat or in human peripheral blood mononuclear cells (PBMCs) to confirm specificity and performance.
• Use appropriate isotype controls and viability dyes in flow cytometry experiments to accurately gate CD5-positive populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-human CD5 monoclonal antibody supports immuno-oncology and immune profiling research, backed by our comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Let CamelBio streamline your raw-material sourcing, from rare targets to large-scale production, accelerating your diagnostic development.