Anti-Human CD45RA Polyclonal Antibody for IF-P - P08575

Core Product Specifications and Parameters

Biological Background: CD45RA Function and Localization

• CD45, encoded by PTPRC, is a receptor-type tyrosine-protein phosphatase essential for T-cell activation through the antigen receptor (PubMed:35767951). Related references: PMID:35767951
• Functions as a positive regulator of T-cell coactivation upon binding to DPP4.
• Contains two PTPase domains; the first has catalytic activity, while the second influences substrate specificity of the first.
• Recruits and dephosphorylates SKAP1 and FYN upon T-cell activation, and modulates LYN activity through dephosphorylation (By similarity).
• Interacts with CLEC10A on immature dendritic cells at the T-cell/APC contact site, raising the T-cell activation threshold to prevent autoreactivity.
• Acts as a receptor for human cytomegalovirus protein UL11, leading to reduced tyrosine phosphorylation and impaired T-cell proliferation upon TCR stimulation.
• Localizes to the cell membrane, membrane rafts, and immunological synapse, consistent with its role in signaling.
• Subject to extensive alternative splicing, generating multiple isoforms with distinct extracellular domains; the immunogen targets isoform 8 (CD45RA), which contains the RA exon.
• Heavily glycosylated and phosphorylated; disease-associated variants can cause severe combined immunodeficiency (SCID).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 10-110) of human CD45RA (isoform 8); perform sequence alignment to confirm specificity for the RA isoform and exclude cross-reactivity with other CD45 variants.
• For immunofluorescence on paraffin-embedded sections (IF-P), standard antigen retrieval protocol may be necessary; we recommend validating on human lymphoid tissue (e.g., tonsil, lymph node) as a positive control.
• Reported cross-reactivity is limited to human; if testing on other species, verify reactivity experimentally.
• As a polyclonal antibody, lot-to-lot variability may occur; we advise titrating and validating each new lot in the intended application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs one-stop access to a comprehensive portfolio of IVD raw materials, technical services, and consulting. Our offering includes validated antibody pairs, optimized polyclonal and monoclonal antibodies for immune cell markers such as CD45RA, and bulk ancillary reagents tailored to your assay development needs. From concept to clinic, CamelBio bridges the gap with reliable supply and expert support for your IVD projects.