Anti-Human CD39/ENTPD1 Monoclonal Antibody for Flow Cytometry - P49961

Core Product Specifications and Parameters

Biological Background: CD39/ENTPD1 Function and Localization

• CD39/ENTPD1 is a membrane-bound ectonucleoside triphosphate diphosphohydrolase that catalyzes the sequential hydrolysis of nucleoside triphosphates (NTPs) and diphosphates (NDPs) to nucleoside monophosphates (NMPs), thereby regulating extracellular nucleotide levels.
  Related references: PMID:8529670 PMID:8626624 PMID:8955160

• By converting proinflammatory ATP and platelet-activating ADP to AMP, CD39 inhibits platelet aggregation and helps maintain blood flow.
  Related references: PMID:8955160 PMID:8996251

• The enzyme localizes to the plasma membrane and caveolae, positioning it to act on extracellular nucleotides.

• CD39 is expressed primarily on activated lymphoid cells, and also found in endothelial tissues, with high expression in placenta, lung, skeletal muscle, and kidney.
  Related references: PMID:7930580 PMID:8955160

• Post-translational modifications include glycosylation and palmitoylation, and the protein contains disulfide bonds; alternative splicing generates multiple isoforms.

• Mutations in the ENTPD1 gene are linked to autosomal recessive hereditary spastic paraplegia 64 (SPG64), underscoring its importance in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen (aa 255-442) covers part of the CD39 extracellular domain, which is accessible for antibody binding in live-cell flow cytometry; ensure that the epitope is preserved under native staining conditions.
• For flow cytometry, titrate the antibody to identify the optimal staining concentration for your cell population, as CD39 expression levels vary between resting and activated cells.
• Include appropriate isotype controls and a viability dye to exclude dead cells that may exhibit non-specific binding.
• When designing multicolor flow cytometry panels, verify that does not overlap with other fluorophores used; consider using single-stain controls for compensation.
• Validate CD39 staining on known positive cells, such as activated lymphocytes or endothelial cell lines, and confirm specificity by correlation with independent CD39 detection methods.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides a streamlined sourcing pathway for CD39/ENTPD1 research, offering an integrated supply of antibodies, ancillary reagents, and technical support. Whether you require validated antibody pairs for assay development, bulk quantities of monoclonal antibodies, or custom sourcing for rare targets, CamelBio bridges your diagnostic needs from concept to clinic. Our expertise in immunology and nucleotide signaling research ensures that you receive reliable and well-characterized raw materials to accelerate your IVD product development.