Anti-Human CD38 Monoclonal Antibody for FC - P28907

Core Product Specifications and Parameters

Biological Background: ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD38) Function and Localization

• CD38 is a multifunctional type II transmembrane glycoprotein capable of synthesizing cyclic ADP-ribose (cADPR), a second messenger that mediates glucose-induced insulin secretion. Related references: PMID:7961800 PMID:8253715

• The enzyme also catalyzes the synthesis of nicotinate-adenine dinucleotide phosphate (NAADP+), a potent Ca²⁺ mobilizer, from 2'-phospho-cADPR and nicotinic acid, as well as from NADP⁺ and nicotinic acid. Related references: PMID:16690024

• As a receptor, CD38 binds to CD31 on endothelial cells, promoting lymphocyte activation, proliferation, and transendothelial migration. Related references: PMID:9551996

• In mature dendritic cells, CD38 regulates CCL21-driven migration, survival, and Th1-polarizing activity. Related references: PMID:16293598

• In lamina propria T lymphocytes, CD38/CD31 interactions activate LCK and LAT signaling, leading to cytokine release. Related references: PMID:11259373

• CD38 is expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, as well as in malignant lymphoma and neuroblastoma.

• The protein undergoes alternative splicing and contains disulfide bonds, glycosylation sites, a signal-anchor, and a transmembrane helix, as annotated by UniProt.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 43–300 of human CD38, which includes the extracellular domain. This design likely favors detection of cell surface CD38 by flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and viability dyes to ensure specific staining and exclude dead cells.
• CD38 expression levels can vary between cell types and activation states; consider using a positive control (e.g., activated lymphocytes, myeloma cell lines) to confirm staining.
• The predicted protein weight is 13kDa/34kDa; note that the band may appear at different molecular weights due to post-translational modifications such as glycosylation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with high-quality IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This anti-CD38 antibody supports immunology and oncology research, and CamelBio offers complementary reagents and technical consulting to streamline your assay development.