Anti-Human CD352/SLAMF6 Monoclonal Antibody for Flow Cytometry - Q96DU3

Core Product Specifications and Parameters

Biological Background: CD352/SLAMF6 Function and Localization

• SLAM family member 6 (SLAMF6), also known as NTB-A, is a self-ligand receptor of the signaling lymphocytic activation molecule family. It modulates activation and differentiation of immune cells, bridging innate and adaptive immunity. Related references: PMID:11489943, PMID:16920955, PMID:22184727
• In natural killer (NK) cells, SLAMF6 triggers cytolytic activity when high surface densities of natural cytotoxicity receptors are present, implicating VAV1 phosphorylation; this activation depends on the adapter SH2D1B/EAT-2 via ITSMs. Related references: PMID:16920955
• In T-cells, SLAMF6 promotes differentiation into a Th17 phenotype, increasing IL-17 secretion through recruitment of RORC to the IL-17 promoter; this costimulatory activity requires the adapter SH2D1A/SAP. Related references: PMID:22184727, PMID:22989874
• SLAMF6 is implicated in thymic NKT cell development, controlling the transition from positive selection to expansion/differentiation, possibly in conjunction with SLAMF1.
• As a negative regulator of humoral immunity, SLAMF6 may inhibit germinal center formation by reducing T-cell:B-cell adhesion, often via increased association with PTPN6/SHP-1 when SH2D1A/SAP is absent.
• Subcellular localization: Cell membrane, single-pass type I transmembrane protein.
• Tissue specificity: Expressed on resting and activated NK cells, T-cells, and B-cells; surface expression is elevated on T-cells from systemic lupus erythematosus (SLE) patients. Related references: PMID:11489943, PMID:22184727
• Post-translational modifications: Phosphoprotein, glycoprotein; contains Ig-like domains, disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 24-215) of human SLAMF6, enabling recognition of surface-expressed receptor; validate staining in relevant live-cell flow cytometry panels.
• As a pre-conjugated antibody, no secondary detection reagent is required; consider titrating the antibody to determine the optimal staining concentration for your cell type and instrument.
• Include appropriate isotype controls and Fc receptor blocking agents to minimize non-specific binding, especially when analyzing Fc receptor-rich immune cells.
• Consider co-staining with lineage markers (e.g., CD3, CD19, CD56) to identify SLAMF6 expression on T, B, and NK subpopulations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive IVD raw-material procurement, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For CD352/SLAMF6 research—central to immunology, autoimmunity, and NK/T cell biology—our portfolio offers ready-to-use flow cytometry tools and customizable reagent solutions to accelerate your assay development and translational projects.