Anti-Human CD34 Monoclonal Antibody (PE/Cyanine7) for Flow Cytometry - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a hematopoietic progenitor cell antigen (also known as GIG3), encoded by the CD34 gene, with a calculated molecular weight of ~40.7 kDa.
• It functions as a potential adhesion molecule, mediating stem cell attachment to bone marrow extracellular matrix or stromal cells, thereby playing a role in early hematopoiesis.
• Acts as a scaffold for lineage-specific glycans, facilitating stem cell binding to lectins on stromal cells and presenting carbohydrate ligands to selectins.
• Located at the cell membrane, with a single-pass transmembrane domain.
• Selectively expressed on hematopoietic progenitor cells and on the small vessel endothelium of various tissues.
• Belongs to the category of glycoproteins and phosphoproteins, and undergoes alternative splicing to produce multiple isoforms.

Experimental Guidance and Technical Tips

• For flow cytometry applications, consider titrating the antibody to determine the optimal concentration for your specific sample type.
• We recommend testing the antibody in relevant human cell lines or primary cells known to express CD34, such as hematopoietic progenitor cells or endothelial cells.
• As the antibody is directly conjugated with PE/Cyanine7, no secondary detection reagent is required.
• To minimize non-specific binding, include appropriate Fc receptor blocking steps and isotype controls.
• Validate staining specificity with a relevant negative control, such as a knockout cell line or cells lacking CD34 expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a leading one-stop sourcing platform for IVD raw materials, providing validated antibodies, antigen proteins, and ancillary reagents to diagnostic manufacturers and research institutions. Our anti-CD34 monoclonal antibody supports research and assay development in hematopoiesis, stem cell biology, and endothelial cell studies. Whether you need bulk monoclonal antibodies, matched antibody pairs, or customized sourcing of rare targets, CamelBio accelerates your project from concept to clinic.