Anti-Human CD34 Monoclonal Antibody () for IF/ICC and FC - P28906

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34 is a hematopoietic progenitor cell antigen, also known as GIG3 and MORT1, encoded by the CD34 gene.
• It functions as a possible adhesion molecule, mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells during early hematopoiesis.
• CD34 may serve as a scaffold for the attachment of lineage-specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.
• The protein presents carbohydrate ligands to selectins, suggesting a role in cell adhesion and migration.
• CD34 is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
• Subcellularly, CD34 is a single-pass type I transmembrane protein located at the cell membrane.
• Post-translational modifications include phosphorylation and glycosylation; alternative splicing produces multiple isoforms.
• The predicted molecular weight is approximately 41 kDa, with observed bands at 35 and 41 kDa likely due to differential glycosylation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 170–290) corresponds to the N-terminal extracellular domain, so the antibody likely recognizes an extracellular epitope, enabling live-cell staining without permeabilization.
• For standard IF/ICC, fix cells with 4% paraformaldehyde; permeabilization is only required if co-staining for intracellular targets.
• In flow cytometry, include appropriate isotype controls and compensation controls for multicolor panels.
• Titrate the antibody to determine the optimal dilution for your specific cell type and assay conditions.
• CD34 expression varies among cell lines and primary cells; validate the antibody in your experimental system with positive (e.g., HUVECs, KG-1a) and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. As an ABclonal partner, we offer ready-to-use CD34 monoclonal antibodies (including fluorescent conjugates like ), validated antibody pairs, and bulk ancillary reagents to accelerate hematopoietic stem cell and vascular biology studies. Our sourcing capabilities extend to rare targets and customized reagent solutions, enabling streamlined IVD assay development and production.