Anti-Human CD32 Monoclonal Antibody for Flow Cytometry - P12318

Core Product Specifications and Parameters

Biological Background: CD32 Function and Localization

• CD32 (FCGR2A) is a low affinity receptor for the Fc region of immunoglobulin gamma (IgG), also known as FcγRIIa.
• Alternative names include CDw32, Fc-gamma RII-a, and FCGR2A1. It belongs to the immunoglobulin superfamily and contains Ig-like domains.
• Upon IgG binding, CD32 initiates cellular responses against pathogens and soluble antigens, primarily by promoting phagocytosis of opsonized particles.
• The receptor is a transmembrane protein located on the cell membrane.
• CD32 is expressed on immune cells including monocytes, neutrophils, and eosinophil platelets.
• The protein is a glycoprotein and phosphoprotein, with a predicted molecular weight of approximately 35 kDa. It undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 36-218), suggesting the antibody is likely to detect surface CD32 on live cells without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Use human monocytes or neutrophils as positive control cells, as they constitutively express CD32.
• Consider Fc receptor blocking and appropriate isotype controls to minimize non-specific staining.
• The conjugate is compatible with standard flow cytometry instruments; verify laser and detector settings for your instrument.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic sourcing partner for IVD manufacturers and research laboratories, offering a comprehensive portfolio of raw materials including monoclonal antibodies, validated antibody pairs, and ancillary reagents. This CD32 antibody supports the development of flow cytometry-based diagnostic assays for immune cell profiling. Our team can assist with sourcing rare target reagents and scaling up supply to meet your workflow needs.