Anti-Human CD31/PECAM1 Monoclonal Antibody for FC - P16284

Core Product Specifications and Parameters

Biological Background: CD31/PECAM1 Function and Localization

• CD31 (PECAM1) is an immunoglobulin superfamily cell adhesion molecule essential for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Phosphorylation of Tyr-690 is critical for TEM and trafficking of the lateral border recycling compartment (LBRC). Related references: PMID:17580308, PMID:19342684
• Through homophilic interactions, it contributes to endothelial cell–cell adhesion at cell junctions. Heterophilic binding with CD177 facilitates neutrophil transendothelial migration. Related references: PMID:27958302, PMID:17580308
• Homophilic ligation of PECAM1 prevents macrophage phagocytosis of viable leukocytes by transmitting a detachment signal, while promoting phagocytosis of apoptotic leukocytes by tethering them to phagocytes. Related references: PMID:12110892
• It modulates bradykinin receptor BDKRB2 activation and regulates bradykinin- and hyperosmotic shock-induced ERK1/2 signaling in endothelial cells. Related references: PMID:18672896
• CD31 induces susceptibility to atherosclerosis, though it does not directly protect against apoptosis.
• The protein is predominantly expressed on platelets, leukocytes, and endothelial cell borders. Isoform Long is the major form; Delta12, Delta14–15, Delta14, and Delta15 show tissue-specific distributions (e.g., Delta15 in brain, testis, ovary, and HUVECs).
• It localizes to the cell membrane, membrane rafts, and cell junctions, consistent with its role in adhesion and signaling.
• Post-translational modifications include palmitoylation, phosphorylation, and N‑linked glycosylation. The protein contains a signal peptide, multiple immunoglobulin‑like domains, a transmembrane segment, and a cytoplasmic tail with ITIM motifs.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 28–601, corresponding to the extracellular domain; the antibody likely recognizes surface CD31, making it suitable for flow cytometry on live cells.
• Titrate the conjugate using relevant positive controls (e.g., HUVECs, platelets, leukocyte subsets) and include an appropriate isotype control.
• For multicolor flow panels, carefully compensate for the signal and avoid fixation protocols that may alter epitope accessibility.
• Validate the antibody in the specific sample matrix (e.g., whole blood, PBMCs) and consider enzymatic or non‑enzymatic cell harvesting methods that preserve surface CD31.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material supply partner, offering one‑stop access to antibodies, antigens, and ancillary reagents for diagnostic development. This anti-CD31 clone is part of our portfolio of optimized monoclonal antibodies for vascular and inflammation research. We support procurement of validated antibody pairs, bulk reagents, and rare‑target sourcing to streamline your assay development workflow.