Anti-Human CD304/Neuropilin-1 Monoclonal Antibody for FC - O14786

Core Product Specifications and Parameters

Biological Background: Neuropilin-1 Function and Localization

• Neuropilin-1 (NRP1, CD304) is a ~103 kDa transmembrane receptor (with smaller isoforms) that binds class 3 semaphorins and VEGF-A165, acting as a critical mediator of axon guidance and angiogenesis. Related references: PMID:9288753, PMID:9529250, PMID:10688880
• NRP1 recognizes a C-end rule (CendR) motif (R/KXXR/K) on ligands such as VEGF-A165, triggering cellular internalization and vascular permeability. Related references: PMID:19805273
• Co-expression of NRP1 with VEGFR2/KDR enhances VEGF-A165 binding and promotes chemotaxis, fine-tuning angiogenic signaling.
• NRP1 modulates mitochondrial iron homeostasis through interaction with the transporter ABCB8/MITOSUR. Related references: PMID:30623799
• Acts as a host factor for SARS-CoV-2 infection by binding the spike protein's CendR motif (RRAR), enhancing viral entry.
• A secreted isoform of NRP1 binds VEGF-A165, potentially sequestering it and inducing apoptosis, and its expression negatively correlates with blood vessel number and integrity. Related references: PMID:10748121, PMID:26503042
• Subcellular localization includes the cell membrane, cytoplasm, mitochondrial membrane, and secreted forms.
• NRP1 is highly expressed in embryonic nervous system and adult heart, placenta, with moderate expression in lung, liver, kidney; also in olfactory epithelium and fibroblasts. Related references: PMID:10688880, PMID:9529250, PMID:33082293, PMID:36213313

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 22-644), making the antibody suitable for detecting surface-expressed NRP1 by flow cytometry without permeabilization.
• For intracellular staining or detection of internalized receptor, a permeabilization step is required.
• The conjugate (equivalent to Alexa Fluor® 594) provides bright, photostable signal with excitation/emission maxima ~590/617 nm; titrate to optimize signal-to-noise ratio.
• Use appropriate isotype controls and avoid repeated freeze-thaw cycles; store at 2–8°C protected from light.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we connect diagnostic developers and researchers with a comprehensive portfolio of validated raw materials for IVD assay development. This anti-CD304/Neuropilin-1 monoclonal antibody, along with complementary antibody pairs, recombinant proteins, and ancillary reagents, supports immunoassay development in angiogenesis, neurobiology, and infectious disease research. Our sourcing expertise ensures rapid access to rare and niche targets, streamlining your path from early-stage R&D to clinical application.