Anti-Human CD303/BDCA-2 Monoclonal Antibody for Flow Cytometry - Q8WTT0

Core Product Specifications and Parameters

Biological Background: CD303/BDCA-2 Function and Localization

• CD303, also known as BDCA-2, dendritic lectin, and CLECSF7, is a member of the C-type lectin family encoded by the CLEC4C gene.
• Serves as a lectin-type cell surface receptor that specifically recognizes non-sialylated galactose-terminated biantennary glycans, binds to serum IgG, and facilitates antigen uptake for peptide presentation to T-cells. Related references: PMID:11748283, PMID:21880719, PMID:25995448
• Plays a role in the inhibition of type I interferon (IFN-α/β) production in plasmacytoid dendritic cells, thereby modulating antiviral innate immunity. Related references: PMID:11748283, PMID:21880719
• Localizes to the cell membrane as a single-pass type II transmembrane protein, anchored via a signal-anchor domain.
• Constitutively expressed in immature monocyte-derived dendritic cells (iMDDC) and significantly down-regulated during LPS-induced maturation, but not by TNF stimulation.
• Contains predicted disulfide bonds and glycosylation sites, contributing to structural stability and lectin domain function.
• May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium upon ligand engagement. Related references: PMID:11748283

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell type and instrument.
• Since CD303/BDCA-2 is a cell surface antigen, standard live-cell staining protocols without permeabilization are appropriate; verify if fixation affects epitope recognition.
• Include appropriate isotype controls and, when possible, use a peptide blocking experiment to confirm antigen-specific binding.
• Cross-reactivity is documented for human; if testing other species, empirical validation is recommended.

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CamelBio supports your dendritic cell and immunology research by providing high-quality IVD raw materials like this anti-CD303/BDCA-2 antibody. In addition to monoclonal antibodies, we offer validated antibody pairs, bulk reagents, and rare-target sourcing to streamline your assay development. Contact us to explore how our end-to-end supply services can accelerate your project from concept to market.