Anti-Human CD30 (TNFRSF8) Monoclonal Antibody () for Flow Cytometry - P28908

Core Product Specifications and Parameters

Biological Background: CD30 Function and Localization

• CD30, also designated TNFRSF8 or Ki-1 antigen, is a member of the tumor necrosis factor receptor superfamily and functions as the receptor for the ligand TNFSF8/CD30L. It plays a role in regulating the growth and transformation of activated lymphoblasts and mediates NF-kappa-B-dependent gene expression. Related references: PMID:8391931
  PMID:8999898
• The protein is a single-pass type I membrane glycoprotein predominantly localized to the cell membrane, with detection also in the cytoplasm.
• CD30 has been detected at the protein level in alveolar macrophages, indicating a role in immune cell function.
• Alternative initiation and splicing generate multiple isoforms, yielding protein weights of approximately 14 kDa, 50 kDa, and 63 kDa (full-length).
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which are essential for receptor stability and signaling.
• The receptor is involved in key cellular processes such as proliferation, differentiation, and apoptosis, particularly in lymphoid cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 19-379 of human CD30, covering the extracellular domain, making this antibody suitable for detecting cell surface CD30 by flow cytometry (FC).
• As a directly conjugated antibody, it can be used without secondary staining; however, titrate the antibody to determine the optimal concentration for your target cell population.
• Validate the antibody in human cell lines or primary samples known to express CD30 (e.g., activated T lymphocytes, Hodgkin lymphoma-derived cells) and include appropriate isotype controls.
• For cell surface staining, ensure cells are viable and use blocking agents to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutions a one-stop sourcing solution for IVD raw materials, from concept to clinic. This anti-CD30 monoclonal antibody supports research in hematological malignancies and immune regulation. Through CamelBio, you can access validated antibody pairs, bulk ancillary reagents, and sourcing for rare targets to accelerate your assay development.