Anti-Human CD3 epsilon Monoclonal Antibody for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 epsilon Function and Localization

• CD3 epsilon is the epsilon chain of the T-cell surface glycoprotein CD3, also known as T-cell surface antigen T3/Leu-4 epsilon chain, encoded by the CD3E gene (also designated T3E) in humans.
• As a component of the TCR-CD3 complex on T-lymphocytes, CD3 epsilon plays an essential role in adaptive immune responses by relaying activation signals from the T-cell receptor into the cell. Related references: PMID:15294938, PMID:15546002, PMID:2470098
• The cytoplasmic domain contains immunoreceptor tyrosine-based activation motifs (ITAMs) that become phosphorylated by Src family kinases LCK and FYN upon TCR engagement, initiating downstream signaling cascades. Related references: PMID:2470098, PMID:40592325
• CD3 epsilon also participates in internalization and down-regulation of surface TCR-CD3 complexes via endocytosis sequences in its cytosolic region. Related references: PMID:10384095, PMID:26507128
• Beyond TCR signaling, CD3 epsilon serves as a receptor for ITPRIPL1, which inhibits T-cell activation by promoting NCK1 interaction and blocking downstream ERK-NFkB signaling and calcium influx. Related references: PMID:12110186, PMID:38614099
• The protein localizes to the cell membrane and contains structural features including a signal peptide, an immunoglobulin-like domain, a transmembrane helix, and conserved disulfide bonds.
• The UniProt record lists molecular weight ~23.1 kDa; post-translational modifications include phosphorylation at ITAM tyrosines, which is critical for signal transduction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–126 of human CD3 epsilon (extracellular domain). For flow cytometry applications, consider a standard cell surface staining protocol using live, unfixed cells to preserve epitope conformation.
• Validate the antibody in a relevant sample system such as human peripheral blood mononuclear cells (PBMCs) or CD3-positive T-cell lines (e.g., Jurkat), ensuring proper isotype control and Fc blocking if necessary.
• Although the antibody is specified for human reactivity, cross-reactivity with other species is not reported; if off-target studies are required, perform sequence alignment and pilot testing.
• Since the product is a rabbit monoclonal IgG, use appropriate anti-rabbit secondary detection reagents when indirect labeling is employed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For projects targeting T-cell biology and immunological disorders, this anti-CD3 epsilon antibody serves as a reliable core reagent for flow cytometry-based assays. With end-to-end support spanning concept to clinic, CamelBio is your strategic procurement partner for rare-target sourcing and custom development needs.