Anti-Human CD3 (CD3E) Monoclonal Antibody for Flow Cytometry - P07766

Core Product Specifications and Parameters

Biological Background: CD3 (CD3E) Function and Localization

• CD3 epsilon chain (CD3E) is a component of the T-cell receptor (TCR)-CD3 complex on T lymphocytes, essential for adaptive immune responses. Related references: PMID:15294938 PMID:15546002 PMID:2470098
• Upon TCR engagement, CD3E is phosphorylated by Src kinases LCK and FYN, creating docking sites for ZAP70 and initiating downstream signaling cascades. Related references: PMID:2470098 PMID:40592325
• CD3E contains immunoreceptor tyrosine-based activation motifs (ITAMs) in its cytoplasmic domain, which are critical for signal transduction. Related references: PMID:2470098 PMID:40592325
• CD3E participates in the internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences in its cytosolic region. Related references: PMID:10384095 PMID:26507128
• CD3E plays an essential role in correct T-cell development.
• CD3E also acts as a receptor for ITPRIPL1, inhibiting T-cell activation by promoting NCK1 interaction and blocking ZAP70 and ERK-NFkB signaling. Related references: PMID:38614099 PMID:12110186
• Subcellular location: CD3E is a single-pass type I membrane protein localized at the cell membrane.
• Keywords: Adaptive immunity, Cell membrane, Disulfide bond, Immunity, Immunoglobulin domain, Membrane, Phosphoprotein, Receptor, Signal, Transmembrane.

Experimental Guidance and Technical Tips

• The immunogen for this monoclonal antibody is a recombinant protein corresponding to human CD3. Validate its reactivity on native human T cells using flow cytometry (FC).
• Optimize the antibody concentration for your specific FC protocol through titration experiments.
• Cross-reactivity has been confirmed only for human. If using samples from other species, verify specificity beforehand.
• Upon arrival, store the antibody under appropriate temperature conditions as recommended for monoclonal antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your streamlined sourcing partner for IVD raw materials, offering efficient access to validated antibodies such as this anti-human CD3 clone for T-cell immunology and immune-oncology applications. We provide bulk monoclonal antibodies, optimized flow cytometry reagents, and ancillary raw materials to support your diagnostic development. For rare or custom targets, rely on our expertise to accelerate your project from concept to clinic.