Anti-Human CD271/NGFR Monoclonal Antibody () for Flow Cytometry - P08138

Core Product Specifications and Parameters

Biological Background: CD271/NGFR Function and Localization

• CD271 (p75NTR, TNFRSF16) is a low-affinity neurotrophin receptor that binds NGF, BDNF, NTF3, and NTF4. It forms a high-affinity proneurotrophin receptor heterodimer with SORCS2 (PubMed:24908487). Alternative splicing generates multiple isoforms. Related references: PMID:24908487
• Plays a dual role in neuronal cell fate, mediating both cell survival and apoptosis during development and in mature neural cells.
• Negatively regulates RHOA activity (PubMed:26646181). Related references: PMID:26646181
• Essential for circadian clock gene oscillation in the suprachiasmatic nucleus and liver, and influences metabolic gene expression (PubMed:23785138). Related references: PMID:23785138
• Cooperates with BFAR to suppress NF-κB and JNK signaling pathways (PubMed:22566094). Related references: PMID:22566094
• Localized to the cell membrane, cytoplasm, perikaryon, and neuronal projections such as growth cones and dendritic spines.
• Exhibits post-translational modifications including glycosylation, phosphorylation, and disulfide bonds, as identified by proteomics and structural analyses.
• Expressed in nervous, adipose, and skeletal muscle tissues; involved in insulin-dependent GLUT4 translocation and glucose uptake (By similarity).

Experimental Guidance and Technical Tips

• The immunogen (aa 29–250) corresponds to the extracellular domain, enabling detection of cell surface CD271 by flow cytometry.
• For FC, use the pre-conjugated antibody; titrate to determine the optimal staining concentration for your cell type.
• Include an appropriate isotype control and Fc receptor blocking step to minimize non-specific binding.
• Validate expression in known positive cell lines (e.g., neuronal or melanoma lines) and consider fixation/permeabilization only if intracellular epitope is targeted.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined one-stop sourcing platform for IVD raw materials, from validated antibody pairs and monoclonal antibodies to bulk ancillary reagents. For research and diagnostic development focused on the neurotrophin receptor CD271, this conjugate is available along with custom procurement support for rare targets, helping you accelerate from concept to clinic.