Anti-Human CD271/NGFR Monoclonal Antibody for FC - P08138

Core Product Specifications and Parameters

Biological Background: CD271/NGFR Function and Localization

• CD271 (p75NTR/TNFRSF16) is the low-affinity nerve growth factor receptor that binds neurotrophins NGF, BDNF, NTF3, and NTF4. It forms a high-affinity heterodimeric receptor with SORCS2 that preferentially interacts with proneurotrophins over mature forms, influencing neuronal development and survival. Related references: PMID:24908487
• Plays a dual role in mediating both cell survival and programmed cell death in neural cells, contributing to the regulation of neuronal populations during development (by similarity).
• Inactivates the small GTPase RHOA, modulating cytoskeletal dynamics and downstream signaling pathways. Related references: PMID:26646181
• Functions in metabolic regulation by facilitating insulin-dependent GLUT4 translocation to the cell surface in adipocytes and skeletal muscle cells, likely via RAB31, thus impacting glucose uptake (by similarity).
• Essential for the circadian oscillation of core clock genes (e.g., BMAL1, PER1, PER2) in the suprachiasmatic nucleus and liver, and for rhythmic expression of metabolic genes in the liver. Related references: PMID:23785138
• Negatively regulates NF-kappa-B and JNK signaling pathways through its interaction with BFAR, providing a control mechanism for inflammatory and stress responses. Related references: PMID:22566094
• Subcellularly localized to the cell membrane, cytoplasm, perikaryon, growth cones, and dendritic spines, reflecting its role in synaptic and structural plasticity.
• Undergoes alternative splicing and post-translational modifications including glycosylation and phosphorylation, features that collectively modulate its stability, trafficking, and signaling competence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 29–250) of human CD271/NGFR, making this -conjugated monoclonal antibody well-suited for live-cell flow cytometry and surface receptor detection.
• For flow cytometry, we recommend performing a titration experiment to determine the optimal antibody concentration. Use human cells expressing CD271 (e.g., neuronal cell lines, immune cells) and include appropriate isotype controls.
• Note that the antibody may recognize both the full-length 45-kDa and shed 35-kDa ectodomain forms; thus, confirm specificity in your model system with appropriate blocking peptides or knockdown controls if needed.
• Although validated for FC, the antibody’s target epitope might be accessible in other surface-based applications such as immunofluorescence on non-permeabilized cells, but users should validate independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing platform for IVD raw materials, supporting diagnostic manufacturers, research labs, and institutes from concept to clinic. For CD271/NGFR—a key receptor in neurobiology, apoptosis, and metabolic regulation—CamelBio can supply validated antibody pairs, recombinant antigens, and ancillary reagents to accelerate your assay development. In addition to this flow-cytometry-optimized monoclonal antibody, we offer sourcing solutions for rare targets, bulk reagents, and tailored technical consultation to meet your project needs.