Anti-Human CD27 Monoclonal Antibody for Flow Cytometry - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Antigen Function and Localization

• CD27, also designated TNFRSF7, is a member of the tumor necrosis factor receptor superfamily and functions as a costimulatory immune checkpoint receptor on T-cells, NK-cells, and B-cells.
• CD70 binding to CD27 promotes antigen-specific T-cell activation and expansion, supporting immune surveillance of B-cells (PubMed:28011863). Related references: PMID:28011863
• Upon ligation by CD70, CD27 activates the TRAF2-PTPN6 axis, which inhibits LCK phosphorylation and drives phenotypic and transcriptional adaptations necessary for T-cell memory (PubMed:38354704). Related references: PMID:38354704
• In early progenitor cells, CD27 engagement provides a negative feedback signal that modulates leukocyte differentiation and hematopoiesis (By similarity).
• The receptor negatively regulates Th2 lymphocyte function in adipose tissue (By similarity).
• CD27 is primarily localized to the cell membrane and is expressed in most T-lymphocytes.
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation, consistent with its role as a transmembrane signaling receptor.
• The protein is associated with apoptosis, disease variants, and has been characterized by direct protein sequencing and proteomics.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell population and instrument.
• CD27 is expressed on T-cells, NK-cells, and B-cells; include appropriate positive and negative controls to verify specificity.
• The immunogen covers the extracellular region (aa 21-192), suggesting recognition of the surface-exposed domain and compatibility with live-cell staining.
• Proper cell handling and gentle centrifugation help maintain surface antigen integrity.
• When designing multicolor panels, consider spectral overlap with the conjugate and combine with other lineage markers.

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