Anti-Human CD244/2B4 Monoclonal Antibody for Flow Cytometry - Q9BZW8

Core Product Specifications and Parameters

Biological Background: CD244/2B4 Function and Localization

• CD244 (also known as 2B4, SLAMF4, NAIL, NKR2B4) is a member of the signaling lymphocytic activation molecule (SLAM) family, a group of receptors that modulate immune cell functions through homo- and heterotypic interactions.
• Acts as an activating natural killer (NK) cell receptor upon binding its ligand CD48, stimulating NK cell cytotoxicity, IFN-gamma production, and granule exocytosis. Related references: PMID:10359122, PMID:11714776, PMID:8376943
• The receptor's function is controlled by cytoplasmic adapters SH2D1A/SAP and SH2D1B/EAT-2; association with SH2D1A and FYN leads to activating signaling involving VAV1, while INPP5D, PTPN6, and PTPN11 mediate signal attenuation in the absence of SH2D1A. Related references: PMID:15713798, PMID:10934222
• CD244 acts as a costimulator in NK cell activation by enhancing signals from other receptors such as NCR3 and NCR1, and also provides costimulatory-like function for neighboring CD8+ T-cells during proliferation. Related references: PMID:10741393
• At early stages of NK cell differentiation, CD244 may function as an inhibitory receptor, potentially ensuring self-tolerance of developing NK cells. Related references: PMID:11917118
• Subcellularly, CD244 localizes to the cell membrane and is enriched in membrane rafts, consistent with its role in immune synapse signaling.
• Expressed broadly in immune tissues (spleen, peripheral blood leukocytes, lung, liver, testis, small intestine) and on all NK cells, monocytes, basophils, TCR-gamma/delta+ T-cells, and a subset of CD8+ T-cells.
• Multiple protein isoforms (30–41 kDa) arise from alternative splicing; the protein is a glycoprotein, phosphoprotein, and contains disulfide bonds and immunoglobulin domains.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 22–221), suggesting the antibody recognizes the native surface receptor, making it suitable for live-cell staining in flow cytometry.
• For flow cytometry, empirically titrate the antibody (e.g., test 0.1–1 µg per 10^6 cells) to determine the optimal concentration for your cell type and experimental conditions.
• CD244 expression levels vary across cell subsets; include appropriate positive (e.g., NK cells) and negative controls, and consider Fc receptor blocking to minimize background.
• Ensure the conjugate is compatible with your instrument’s laser and filter configuration (excitation ~590 nm, emission ~617 nm).

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